Per3 Gene and Diurnal Preference
The high base solution will rupture the cell membrane and create pores in the membrane. Then 100µl of 0.1M HCl and 100µl of 1M Tris – HCl was added at a pH of 7. The cell organelles are present in the solution and they are separated from the solution by centrifugation. The crude cell lysate will contain the DNA.