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Biology Lab Report

Biochemical identification of unknown bacteria
After incubation growth was observed visually. Flocculent growth was observed having cloudy appearance while shaking. Sediments were found to be granular.To investigate oxygen requirement of given unknown organism thioglycollate medium was used. Thioglycollate and cystine (major constituent of media) provides reducing environment and higher viscosity restricts oxygen penetration in to the tube. Thus, the growth of organism can be monitored at different level as per their oxygen requirement.
Pages: 4 (1000 words), Lab Report
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Calculation for RBC
Fourth sound is heard at 10mm above diastolic pressure while the last korotkoff sound is heard at the precise diastolic pressure.Oscillometric principle :Another method of measuring blood pressure. It makes use of the same instrument but in addition uses a transducer which gives the oscillation readings of the blood flow. As the pressure in the cuff varies with simultaneous expansion and contraction of brachial artery an oscillation is produced.Hypertension :A medical phenomenon indicating an
Pages: 1 (250 words), Lab Report
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Oldham Pond study
Haledon at an interval of three weeks; the first data being collected being collected on 4th February, 2012 and the second data was collected on 25th February, 2012. The study area is shown in figure 1 in the appendices page.The data collected was on temperature, dissolved oxygen, conductivity, Ph and the total alkalinity. Moreover, the prevailing weather conditions were noted including the temperature and the wind conditions. During the first visit, data was taken on at the surface and at a
Pages: 5 (1250 words), Lab Report
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Darwinian Snails
Blue snails with shell thickness of 6.0 and 7.0, five yellow-green snails with 2 having shell thickness of 3.0, another 2 with thickness 5.0 and 1 with shell thickness of 4.0, and one 0range-yellow snail with shell thickness of 10. Tank two also had same number of snails that comprised of three blue with shell thickness of 4.0, and 8.0, one orange with shell thickness of 9.0 and four yellow-green with shell thickness of 3.0, and 5.0, I also included one crab in the tank.In tank 3, I added two
Pages: 7 (1750 words), Lab Report
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Breast Cancer
The cell line is 8.26 while for the sixth cell line is 29.Therefore, it is clearly evident that the color intensity is higher in the sixth cell line than the fifth cell line. According to studies, it is known that glycosylation is more pronounced in the breast cancer cells (Hakomori 1985). The reason for this is because of the increase in the oligosaccharide structures
Pages: 4 (750 words), Lab Report
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Writer decided the title
In the experiment, the expression of VVA reactive glycol-epitopes was investigated with the use of human breast cancer cell line SK-BR-3 (The fourth cell line).The presence of T and Tn glyco-epitopes in terms of VVA bindings seems to be unlikely because of the labelling seen in some few cells.This is supported by Kawaguchi et al(2006) who found out that there is no correlation between the VVA-reactive oligosaccharides expression and various metastatic potential indicators like oestrogen
Pages: 2 (500 words), Lab Report
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Regulatory Genes
In this case, it is as well known that LHX3 plays a role in a cell by binding selected proteins referred to as protein partners and they include NLI and Pit-1.Mutations in the LHX3 gene have been seen to be related with novel,severe form of CPHD(Netchine et al. The hypothesis tested for the first experiment is mutations in the LXH3 gene can interfere with LHX3a and LHX3b’s ability to bind to DNA promoters and reporter genes. The hypothesis of the second experiment is mutations in the LHX3
Pages: 4 (1000 words), Lab Report
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Bacterial transformation and gene expression
(Jeffery et al. Plasmids are very advantageous for the genetic engineering. Plasmids code for many antibiotic regions and they have the ability to accept the gene of interest. The transformation of our gene of interest into the plasmid is called recombination and the bacteria are called recombinant bacteria. Thus plasmids can be used as cloning vehicles or vectors. These plasmids are not essential for the survival of bacteria, but in some instances, for survival in the different environments,
Pages: 8 (2000 words), Lab Report
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Testing of biomolecules
This report will therefore compile various methods that have been developed in the testing of biomolecules and depict the importance of each in biochemistry.The biomolecules that we shall look at include; - carbohydrates-three tests including the test for reducing sugars, test for non-reducing sugars, proteins, the tests analyzed will check for the presence of amino acids in the given samples. Amino acids are the building blocks for proteins and they are tested using the biuret method (John and
Pages: 5 (1250 words), Lab Report
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Per3 Gene and Diurnal Preference
The high base solution will rupture the cell membrane and create pores in the membrane. Then 100µl of 0.1M HCl and 100µl of 1M Tris – HCl was added at a pH of 7. The cell organelles are present in the solution and they are separated from the solution by centrifugation. The crude cell lysate will contain the DNA.
Pages: 7 (1750 words), Lab Report
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LABORATORY EXERCISE 6 Enzyme activity
A plastic tub was then filled with cold tap water to an approximate depth of 3 to 4 inches after which the temperature of the water was adjusted to between 37 and 40 oC using warm water. 50ml graduated cylinders were then prepared by submerging them in the water tubs and allowing them to fill without any air bubbles. The cylinder was then put upside down with its mouth submerged. The cylinder was then put in a ring stand and secured in position with the mouth just below the surface of the
Pages: 8 (2000 words), Lab Report
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Interspecific and Intraspecific Plant Competitions
Some of these interactions include depletion of resources, use of space as well as production of toxins. Other forms of interactions include overgrowths whereby some species grow over others thus depriving them of requirements for growth including light needed for photosynthesis (Tow and Alec, 35). In that case, such plants are suppressed and in the long term, become eliminated by the superior plants that mounted on them. Other forms of interactions include territorial interactions. This form
Pages: 8 (2000 words), Lab Report
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Catabolite Repression and Induction of Beta-galactosidase Synthesis in E. coli
Apart from induction, synthesis of β-galactosidase is also influenced by catabolite repression. The process involves slow down of the synthesis process, facilitated by lactose, especially when a presence of glucose is detected. Intuitively, glucose acts as a better energy and carbon source than lactose (Wallenfels, 1972, 67). 
Pages: 6 (1500 words), Lab Report
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Main Methods of Successful Transformation of Bacterial Cells with Different Plasmid
According to Piddock (2014), conjugation happens when cells with plasmid produce pili, which is a tube for attachment to cells without the plasmid hence making the transfer of plasmid possible. Transformation takes place when cells treated with electric or chemicals substances are induced to uptake a naked DNA molecule.
Pages: 6 (1500 words), Lab Report
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The writer names the report
Various physiological and anthropometrical parameters were measured from male college students (Appendix- Excel Sheet attached) through standard protocol as per VLE. The subjects were selected on the basis of random sampling to reduce the chances of bias in our study. The inclusion criteria for our study were male college students in the age group of 19-24 years, non-smokers and must be involved in some level of physical activity. The exclusion criteria were male students aged below 19 years
Pages: 6 (1500 words), Lab Report
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Lactase Enzyme Activity
Based on my knowledge of the effects of temperature and pH on the enzymatic activity of lactase, if the lactase used in today’s lab was extracted from human cells, I hypothesize that lactase enzyme activity will be optimal at temperature and pH of 400C and 8 respectively.
Pages: 6 (1500 words), Lab Report
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Alcoholic Fermentation
Beer preparation by fermentation involves picking rye, wheat or barley followed by germinating, drying and pulping it into a mash. The mash is mixed together with hot water and transferred to a fermentation vessel to commence the process of fermentation. Yeast is added to the mixture that converts the sugar present in the ash to carbon dioxide and alcohol. 
Pages: 5 (1250 words), Lab Report
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Staphylococcus and Streptococcus
A petri dish containing 1 TSA 5% sheep blood agar was divided in half. A sterile swab was used to swab the throat and skin. A streak inoculation was performed for each sample on each section of the plate, which was incubated for 24 hours at 37 oC. The resultant colonies were then tested for Gram staining and the presence of alpha, beta, and gamma hemolytic bacteria. This test was performed by observing the presence or absence of red blood cell hemolysis on sheep blood agar. The next test that
Pages: 4 (1000 words), Lab Report
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