The juice is then treated with chemicals to kill any fungi which is available in it. It is at this stage, where HPLC finds its use. This is because some of the chemicals always remain in the final product. In connection to this, HPLC is always used to determine, if there are any traces of the chemicals in the final sugar. If there are any, it is then taken back for treatment (Scoog &Holler, 1992).The main limitation of this technique is that it cannot be used for very complex chemical elements. In addition, the technique cannot detect very small amounts of elements. Finally, in cases where there is a reversible solvent matrix, it may not be able to identify the chemicals present in the sample (Kebbekus & Mitra, 1998).AAS is a technique used to identify, and quantify elements. It has a source of radiation, which is used to excite the elements in the sample. During excitation, the elements will absorb a specific amount of energy, to go to the next electron levels. Hence the name absorption spectroscopy (Ballinger et al.The amount of radiation that is absorbed by the element, can then be used to identify it. This is done using a set of tables, which has all the data regarding all the elements and the amount of energy that they absorb (Scoog & Holler, 1992).AAS technique has found applications in various sectors .For example, in water analysis, where it is used to determine the amount of dissolved metals. It is also used in clinical analysis, to determine the amount of heavy metals in blood, urine and in food. Finally, it is also used in the pharmaceutical industries to determine, if there is any residue of the enzymes which was used to produce the drug (TM, 2000).In order for a sample to be analyzed, it has to be atomized, then a radiation is passed through the sample, after the atoms has been excited the energy released is then passed through a monochromator so as to distinguish the different radiations (TM, 2000).AAS is used in many industries as stated above. For example, in the distillation of water. According to many authors, water always contain, dissolved metals, suspended solids and other dissolved elements. In connection to this, many industries prefer using AAS, because it does not require a lot of sample preparation. The only thing, which one has to do is to take the water the way it is, and analyze it. Then, compare it with a reference material to see the chemicals present in the water
Handbook, Fifth Edition. McGraw-Hill Professional
Test Methods: Methods for Organic Chemical Analysis of Municipal and Industrial Wastewater. (2000) U.S. EPA-600/4-82-057.
Kebbekus, B. and Mitra, S. (1998) Environmental Chemical Analysis. New York, NY: Chapman & Hall.
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