The treated cells were used as donor nuclei for nuclear transfer. The transferred cell was passed through electric pulses that were an activation factor. This gave it a high multiplication rate of 62.5% twelve hours after activation. The nuclear transfer and exposure to electric pulses led to condensation of chromosome by 0.5% due to the exposure of the cell to the colchicine solution, most of the embryos were diploid. After two hours of the fusion, there was detection of very strong fluorescence in most of the reconstructed oocytes. These reconstructed embryos later developed to blastocyst phase that most of them expressed Enhanced Green Fluorescent Protein.These embryos were later transferred into surrogate gilts that out of four of them, three were found to be pregnant. One of the pregnant surrogates gave birth into the healthy piglets with characteristics of showing that they contain Enhanced Green Fluorescent Protein.Phytase that is very much effective in digestion of phytate is expressed in the salivary glands parotid secretory organs of transgenic pigs. The salivary glands are found in the mouth on the mouth. The scientist made sure that phytase is produced early enough during the start of digestion so that to give it time for distention as food moves down the intestines.How they made it: it was developed by introducing the transgene pigments which were composed of some promoters elements of murine parotid secretory protein gene a bacterial by the name Escherichia coli which is responsible for phytase genes (Golovan et al, 167). This changed its genetic characteristics into a fully fertilized porcine embryo by pronuclear. The embryo together with others was through surgical method implanted into reproductive track of a uterus synchronized female.The pigs have shown some good characteristics of well-growing habit. They needed little-supplemented feed as compared to the Yorkshire breed. Compared to Yorkshire, they could eat the same diet but more supplemented with P for Yorkshire.However, there have been some emerging problems on their growth. For example, a breed from transgenic has shown characteristics of weak legs. This is the effects of over expression of the genes. The same fact has caused weaknesses in the reproductive system of the breeds (RosENGARD 207). On the other hand, under expression of the genes has
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