Differential diagnosis could be replaced by reverse transcription polymerase reaction, DNA sequencing and DNA microarray.Quantitative real time reverse transcription polymerase chain reaction (RT-PCR) can be used for differential expression of genes in HGDN and WD-HCC. This method is fast, highly sensitive and specific. The starting material could be liver biopsy or surgical specimen. Identification of lymphomas by demonstration of presence or absence of clonality is done suitaby using PCR. The most efficient markers of HCC are used to establish a specific molecular diagnostic index. The selected genes cover the most relevant metabolic pathways involved in liver carcinogenesis and are regulated at the transcriptional level (Paradis, et al. Quantitative real time PCR measure microMRNA which make useful biomarkers in differential diagnosis of HCC .DNA microarrays is important in distinguishing different histological grades of HCC, and genetic profiles associated with venous invasion and metastasis. The microarray technology enables identification of specific gene expression patterns that distinguish HCC. Hsp 70 and Hsp 90 families are molecular chaperones that regulate many key components of apoptotic pathways and molecular chaperones. To clarify the characteristic changes associated with the multistep process, the gene expression of 7 early components and 7 progressed components of nodule in nodule type HCC are analyzed. Hsp 70 in particular is a sensitive marker for the differential diagnosis of early HCC. The expression of Hsp 70 increases in early and progressed HCC (Chuma, et al.Genes that were classified in the immune system detoxification category were down regulated in carcinogenic cells. In the early stage of HCC development, immune surveillance deteriorates and multiple apoptosis and histocompatibility complex related genes were down regulated during the transition of liver cirrhosis to GI or GII HCC. There are a series of genes classifiers with an accuracy of 95% distinguishing LGDN and HGDN. In addition, these gene classifiers can effectively differentiate between GI-GII and GII-GIII HCC. In moderately to poorly differentiated HCC, the reduced rate of apoptosis could indicate major tumor progression characteristic (Maass, et al.DNA sequencing is a non-invasive, early detection and differential diagnosis of HCC. The method has
Chuma, M. et al., 2003. Expression Profiling in Multistage Hepatocarcinogenesis: Identification of HSP70 as a Molecular Marker of Early Hepatocellular Carcinoma. Hepatology, 37(1), pp. 198-207.
Jemal, A. et al., 2011. Global Cancer Statistics. CA Cancer Journal Clinics, Volume 61, pp. 69-90.
Kan, Z., Zheng, H., Liu, X. & Li, S., 2013. Whole genome sequencing identifies recurrent mutations in hepatocellular carcinoma. Genome Research, 23(9).
Kudo, M., 2013. Early Hepatocellular Carcinoma: Definition and Diagnosis. Liver Cancer, 2(2), pp. 69-72.
Luo, Q. et al., 2015. Leukemia inhibitory factor receptor is a novel immunomarker in distiction of well-differentiated HCC from dysplastic nodules. Oncotarget, pp. 1-11.
Maass, T. et al., 2010. Microarray-Based Gene Expression Analysis of Hepatocellular Carcinoma. Current Genomics, 11(4), pp. 261-268.
Paradis, V., Bieche, I. & Bedossa, P., 2003. Molecular Profiling of Hepatocellular Carcinomas (HCC) Using Large-Scale RealTime RT-PCR Approach. The American Journal of Pathology, 163(2), pp. 733-741.
Qian, J. et al., 2010. Characteristics of Hepatic IGF-II Expression and Monitored Levels of Circulating IGF-II mRNA in Metastasis of Hepatocellular Carcinoma. American Journal of Clinical Pathology , Volume 134, pp. 799-806.
Xu, H. et al., 2015. Non-invasive Analysis of Genomic Copy Number Variation in Patients with Hepatocellular Carcinoma by Next Generation DNA Sequencing. Journal of Cancer, 6(3), pp. 247-53.
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